Indonesia Conference Directory

Corresponding Author
Eka Prasasti Nur Rachmani

Institutions
Department of Pharmacy, Faculty of Health Sciences, Jenderal Soedirman University, Purwokerto, Central Java, Indonesia

Abstract
Slatri leaves (Calophyllum soulattri) which contains flavonoids, tanin, xhantone, and cumarin, has potential as an antioxidant. This research objective is to find antioxidant activity and total flavonoid total extract and fraction of slatri leaves. This research was conducted with laboratory experimental methods which include antioxidant activity assay using DPPH (2,2-diphenyl-1-pikrilhidrazil) is determined by the amount of DPPH radical absorption barriers represented by IC50. Levels of total flavonoids was determined by colorimetric method with AlCl3 reagent routinely expressed as a percentage in the extract. The results showed IC50 leave ethanol extract against DPPH with slatri of 39.63 ± 14.99 ppm and total flavonoid content of leaf extracts slatri of 25.677 ± 0.046% in the extract routine

Keywords
Antioxidants, Slatri (Calophyllum soulattri), DPPH, total flavonoids, Colorimetric

Topic
Pharmaceutical Sciences

Link: https://ifory.id/abstract/EgxjmGNqMwJu

Corresponding Author
muhamad fareza

Institutions
1 Pharmacy Department, Universitas Jenderal Soedirman
2 Chemsitry Department, Universitas Padjajaran

Abstract
Background : The disscovery for new anticancer agents is one of the important research. This is because the existing chemotherapy drugs that are no longer effective, because its resistance and side effects, in treating cancer. Various approaches are carried out in finding new anticancer drug. One approach to obtaining these new active compounds as anticancer agent are from the plants.Chisocheton is a plant genus from Meliaceae familiy known as rich source of terpenoid compounds which potential ones as anticancer drug candidate. Methods : Isolation of sesquiterpenoid from stem bark of C. microcarpus were carried out by extraction and chromatographic methods. The determination of the compound structure were analyzed based on spectrum data of NMR (Nuclear Magnetic Resonance) and HRMS (High Resolution of Mass Spectroscopy). Cytotoxicity of this compound was evaluated with MTT assay. Results 10-hydroxyaromadendrane was isolated as colorless oil. Its molecular formula, C15H26O, was established from the quasi-molecular ion peaak at m/z 223.2064 [M+H]+ (calculated for C15H27O , 223.2062) in HR-ESI-MS spectrum. From 1H-NMR spectrum, its showed four methyl signal (δ 0.82, 0.89, 0.92, 1.04 ppm), four methylene signal (1.15, 1.29, 1.49, and 1.59 pp) , and five methine signal (0.01, 0.51, 1.69, 1.72 and 1.86 ppm). Its 13C-NMR spectrum indicated four methyl signal (δ 16.10, 16.30, 28.67 and 37.78 ppm), four methylen signal (δ 37.78, 29.11, 25.78, 18.81 ppm), five methine signal (δ 58.25, 39.74, 38.46, 28.57 and 22.32 ppm) and two quartener carbon signal at δ 74.60 (Cq-O) and 18.39 ppm (Cq). This compound showed cytotoxic activity against MCF-7 breast cancer cells with IC50 value of 597,13 µg/mL. Conclusion: A aromadendrane type-sesquiterpenoid has been isolated from the stembark of C. microcarpus. Based on the spectroscopic evidance, the compound was 10-hydroxyaromadendrane. This compound showed weak cytotoxicity against MCF-7 breast cancer cells with IC50 value of 597,13 µg/mL.

Keywords
Chisocheton microcarpus, Aromadendrane, MCF-7

Topic
Pharmaceutical Sciences

Link: https://ifory.id/abstract/evkQbNfyZG6V

Corresponding Author
Tuti Sri Suhesti

Institutions
Department of Pharmacy
Faculty of Health Sciences
Jenderal Soedirman University,

Abstract
Most free drugs are characterized by high liposolubility, but they often have limited clinical applications because of poor dissolution and poor bioavailability. The purpose of present study was to evaluate commercial preparations of Acetaminophen tablets with respect to drug release, Pharmaceutical formulations often require additional materials (excipients) in addition to the active ingredient itself. The use of excipients in tablet formulations is expected to produce a quality tablet of Acetaminophen which meets the requirements of the physical properties of tablets and is able to provide good drug release. The result showed that commercial preparations of Acetaminophen vary widely in their dissolution behavior in dissolution run experimentation. Identifying this behavior of the products was essential as an in vitro tool for screening a good and a bad formulation. Parameter test drug levels observed were dissolved at t = 30 min (C30) with default parameters standard C 30 shows the results should not be less than 80% of drug levels. The different dissolution profiles of 8 samples were showing the results of dissolution with C30 are eligible (> 80%).

Keywords
bioavailability, release, dissolution, acetaminophen

Topic
Pharmaceutical Sciences

Link: https://ifory.id/abstract/NvwPZQhGJrpH

Corresponding Author
Haryati Haryati

Institutions
1Medical Faculty of Halu Oleo University, Kendari, South East of Sulawesi, Indonesia
2Nursing Concentration of Medicine Faculty of Halu Oleo University, Kendari, South East of Sulawesi, Indonesia

Abstract
Background: Wounds are injuries to parts of the body, when the skin and underlying tissue lose their tissue continuity. Antimicrobial preparation such as povidine iodine is commonly used to reduce the risk of infection in minor injuries, but it can cause side effects. Lemongrass is one of the grass plants often used as a medicine. It has broad indications as analgesic, antiseptic, antiemetic, antitussive, antirheumatic, and anticonvulsant. Lemongrass is also used as an antibacterial, anti-inflammatory, and antioxidants that can affect the wound healing process. Purpose: This study aims to determine the effectiveness of lemongrass extract (Cymbopogon citratus) 30%, 60 and 80% compared with negative control and positive control group on incised wound healing in white mice. Methods: This study is a true experimental research with post-test only control group design using complete randomized. This research used 25 white mice divided into 5 groups: 30% lemongrass extract, 60% lemongrass extract, 80% lemongrass extract, negative control (Aquades) and positive control (Bioplacenton). Result: Analysis of Shapiro Wilk normality test data show p value > 0.05. Levenes Test homogeneity test was obtained p = 0.730 (p> 0.05), it showed that homogeneous data. Based on One Way ANOVA test showed result with p value > 0.05 (0.881). It means no difference the healing of incision wound. But for the average wound healing time it is known that lemongrass extract 60% is more effective in healing wounds in white mice compared to the concentration of lemongrass extract 30% and 80%, where total wound healing occurred on day 14 in the 60% lemongrass extract. Conclusion: There is no difference in effectiveness of lemongrass extract (Cymbopogon citratus) 30%, 60% and 80% compared with negative control groups and positive control on incision wound healing in white mice.

Keywords
incision wound, lemongrass extract, white mice, wound healing

Topic
Pharmaceutical Sciences

Link: https://ifory.id/abstract/Xnwp6tAeQKxk

Corresponding Author
Warsinah warsinah

Institutions
Department of Pharmacy, Faculty of Health Science, Jenderal Soedirman University

Abstract
Bandotan (Ageratum conyzoides L) herbaceous plants have been empirically used for wound medicine and digestive disorders. The treatment is related to its pharmacological activity as an antibacterial. The purpose of this study was to isolate responsible compounds as antibacterial. The research phase is extraction using maceration method, fractionation by liquid vacuum column chromatography followed by TLC, the same profile of TLC combined and then tested for its activity against Esceresia coli and Staphylococus aureus. The results of the antibacterial activity test showed that the inhibition of ethyl acetate extract was 14 mm, the combined fraction 4 was 16 mm and subfraction Fg4.2 was 14.5 mm at S aureus, whereas in E coli extract the ethylacetate extract was 11 mm, the combined fraction 4 was 13 mm and sub Fg4.2 of 10 mm. The minimum inhibition of combined Fg1.2 subfraction against S. aureus is 12,5 mg / ml and 25 mg / ml for E. coli. Identification results with UV-vis, FT-IR and GC-MS showed that in bandotan herbs contain compounds called coumarin, kromen, fatty acids and eicosan.

Keywords
Ageratum conyzoides, isolation, Bioassay guided fractionation

Topic
Pharmaceutical Sciences

Link: https://ifory.id/abstract/9LXyv2nf3wUj

Corresponding Author
Nia Kurnia Sholihat

Institutions
Department of Pharmacy, Faculty of Health Sciences, Universitas Jenderal Soedirman
Purwokerto, Indonesia
*corresponding author: nia.sholihat[at]unsoed.ac.id

Abstract
There have been many studies evaluating patient satisfaction with pharmacy services in Indonesia. However, the results remain unclear and vary. Therefore, we conduct a systematic review to observe levels of satisfaction on pharmacy service and factors associated with patient satisfaction in community settings in Indonesia. We used Google Scholar, Portal Garuda, and Neliti as main databases. The date of the last search was May 2019. Inclusion criteria used in this study as follows: (1) original study, (2) measure patient satisfaction on pharmacy service, (3) settings in community pharmacies or community health centers, (4) published in journals or proceeding, and (5) online full text available. We used keyword both in Bahasa and in English in each database. Of the 9,617 studies retrieved through Google Scholar search (9560 studies), Portal Garuda (19 studies), and Neliti (38 studies), 34 records were excluded because of duplication. Of the 44 studies included in the full text review, 38 studies met the eligibility criteria and were included in qualitative synthesis. The most frequently used instrument has been SERVQUAL questionnaire (68.42%), covering 5 dimensions: reliability, responsiveness, assurance, empathy, and tangible. Twenty-four studies showed that patients were satisfied with pharmacy service. Only 4 studies showed that patients were not satisfied, mostly took place in primary health centers. Factors which showed a significant relationship with satisfaction were pharmacy appearance, drug information service, availability and variety of drugs, speed of service, age, education, employment status, quality of service, and price of drugs. Interestingly, 4 studies pointed that the implementation of pharmaceutical care showed no significant relationship with patient satisfaction. This study suggested pharmacists should promote their existence through delivering more pharmaceutical care to the patients.

Keywords
satisfaction, pharmacy service, community, Indonesia

Topic
Pharmaceutical Sciences

Link: https://ifory.id/abstract/Q9LYmR3vtk4N

Corresponding Author
Heny Ekowati

Institutions
Pharmacy Department, Health Sciences Faculty, Universitas Jenderal Soedirman,
Jl. Dr. Soeparno, Karangwangkal, Purwokerto 53123

Abstract
Colon cancer is one of the most common cancer in the world. Recently, natural products were used widely as complementer therapy for colon cancer. Previous study reported Nigella sativa has in vitro chemopreventive activity. This study aimed to observe the effect of cloroform extract of Nigella sativa seed (NSS) on rat colon cell after initiation of 7,12-dimethylbenz [a] anthracene. An experimental study design was set up with post only control group design using rats. Rats were divided into five groups, 12 rats in each group: Group I, the control group was given DMBA orally 20 mg/kgBW twice a week for five weeks, group V is the solvent control group was given corn oil. The other three groups were given DMBA + NSS, at the dosage of 250 mg/kgBW, 500 mg/kgBW and 750 mg/kgBW. NSS extract was dissolved in corn oil and administered daily per oral during the next two weeks before and during the initiation of DMBA. At the end of the experiment (16 week) all rats were sacrificed. Observations of colonic cell proliferation was performed using hematoxylin and eosin staining (H&E) and AgNOR. Data were analyzed using one-way ANOVA, followed by Tukey HSD. H&E staining showed that necrosis activity was lower in treated groups compared to DMBA group. AgNOR staining showed mAgNOR was significantly decrease following the increasing dose of NSS (250 mg/kgBW, 500 mg/kgBW and 750 mg/kgBW) were subsequently 1.62 ± 0.086, 1. 60 ± 0.101 and 1.39 ± 0.049 (p<0.05). The results showed that NNS reduce the damage of colon cells and inhibit colon cell proliferation in DMBA induced rats. This study indicated that Nigella sativa can be developed into chemopreventive agent for colon cancer.

Keywords
Nigella sativa, colon cancer, DMBA, in vivo

Topic
Pharmaceutical Sciences

Link: https://ifory.id/abstract/rzMn9atkTCvD

Corresponding Author
Talha Bin Emran

Institutions
(1) Department of Pharmacy, BGC Trust University Bangladesh, Chandanaish, Chittagong-4381, Bangladesh
(2) Department of Biochemistry and Molecular Biology, University of Chittagong, Chittagong-4331, Bangladesh

Corresponding author: *Talha Bin Emran PhD, Assistant Professor, Department of Pharmacy, BGC Trust University Bangladesh, "BGC Biddyanagar", Kanchannagar-4381, Chandanaish, Chittagong, Bangladesh. E-mail: talhabmb[at]gmail.com or talha-bgctub[at]bgctrustbd.org, Tel: +88-01819942214

Abstract
Background: Urena sinuata L. is a wild shrubby plant with some folk medicinal use. To our knowledge, the biological importance of this plant has not been investigated yet. Purpose: The present study aimed to determine the neuropharmacology, antinociceptive, anti inflammatory and antipyretic effects of the chloroform extract of Urena sinuata leaves CEUS in rodents and to elucidate the possible mechanism of antinociception involved with its acute toxicity and phytochemical studies. Methods: Neuropharmacological activities of CEUS were conducted by hole cross, open field test, elevated plus maze test and thiopental induced sleeping time test. For the analgesic activity of CEUS different methods like hot plate test, acetic acid induced test, formalin induced test, tail immersion test and glutamate induced nociception were used. Additionally, the possible mechanism of nociception is identified by cyclic guanosine monophosphate and ATP sensitive K channel pathway analysis. Carrageenan induced rat paw edema and cotton pellet induced granuloma test also were used to detect anti inflammatory activity and brewers yeast induced pyrexia test for antipyretic activity. Results: The extract 200 and 400 mg/kg was administered orally 60min prior to subjection to the respective test. The results obtained demonstrated that CEUS produced significant neuropharmacological, anti inflammatory and antipyretic activity with low or no toxicity. The extract also exerts antinociceptive response in all the chemical and thermal-induced nociception models. Furthermore, it involves cyclic guanosine monophosphate and ATP sensitive K channel pathway mediated antinociceptive effect. Conclusions: These data show for the first time that CEUS has significant neuropharmacological, anti inflammatory and antipyretic effects which appear to be related to the inhibition of the glutamatergic system and rationalized the traditional use of the leaf in the treatment of different types of inflammation in intestines and bladder. Thus the leaves of Urena sinuata could be used in the treatment of several types of inflammation in intestines and bladder.

Keywords
Urena sinuata, Malvaceae, Neuropharmacological activity, Antinociceptive, Anti-inflammatory and Antipyretic

Topic
Pharmaceutical Sciences

Link: https://ifory.id/abstract/BGLnU3Efu4Dh